Isolation and characterization of a bat SARS-like coronavirus that uses the ACE2 receptor.
Identifieur interne : 001908 ( Main/Exploration ); précédent : 001907; suivant : 001909Isolation and characterization of a bat SARS-like coronavirus that uses the ACE2 receptor.
Auteurs : Xing-Yi Ge ; Jia-Lu Li ; Xing-Lou Yang ; Aleksei A. Chmura ; Guangjian Zhu ; Jonathan H. Epstein ; Jonna K. Mazet ; Ben Hu ; Wei Zhang ; Cheng Peng ; Yu-Ji Zhang ; Chu-Ming Luo ; Bing Tan ; Ning Wang ; Yan Zhu ; Gary Crameri ; Shu-Yi Zhang ; Lin-Fa Wang ; Peter Daszak ; Zheng-Li ShiSource :
- Nature [ 1476-4687 ] ; 2013.
Descripteurs français
- KwdFr :
- Animaux, Cellules Vero, Chine, Chiroptera (virologie), Données de séquences moléculaires, Fèces (virologie), Glycoprotéine de spicule des coronavirus (), Glycoprotéine de spicule des coronavirus (métabolisme), Génome viral (génétique), Humains, Pandémies (), Pandémies (médecine vétérinaire), Peptidyl-Dipeptidase A (génétique), Peptidyl-Dipeptidase A (métabolisme), Pénétration virale, Réaction de polymérisation en chaine en temps réel, Récepteurs viraux (génétique), Récepteurs viraux (métabolisme), Réservoirs d'agents pathogènes (virologie), Spécificité d'espèce, Spécificité d'hôte, Syndrome respiratoire aigu sévère (), Syndrome respiratoire aigu sévère (médecine vétérinaire), Syndrome respiratoire aigu sévère (transmission), Syndrome respiratoire aigu sévère (virologie), Technique d'immunofluorescence, Virion (isolement et purification), Virion (ultrastructure), Virus du SRAS (génétique), Virus du SRAS (isolement et purification), Virus du SRAS (métabolisme), Virus du SRAS (ultrastructure), Viverridae (métabolisme).
- MESH :
- génétique : Génome viral, Peptidyl-Dipeptidase A, Récepteurs viraux, Virus du SRAS.
- isolement et purification : Virion, Virus du SRAS.
- médecine vétérinaire : Pandémies, Syndrome respiratoire aigu sévère.
- métabolisme : Glycoprotéine de spicule des coronavirus, Peptidyl-Dipeptidase A, Récepteurs viraux, Virus du SRAS, Viverridae.
- virologie : Chiroptera, Fèces, Réservoirs d'agents pathogènes, Syndrome respiratoire aigu sévère.
- Animaux, Cellules Vero, Chine, Données de séquences moléculaires, Glycoprotéine de spicule des coronavirus, Humains, Pandémies, Pénétration virale, Réaction de polymérisation en chaine en temps réel, Spécificité d'espèce, Spécificité d'hôte, Syndrome respiratoire aigu sévère, Technique d'immunofluorescence, Virion, Virus du SRAS.
- Wicri :
- geographic : République populaire de Chine.
English descriptors
- KwdEn :
- Animals, China, Chiroptera (virology), Chlorocebus aethiops, Disease Reservoirs (virology), Feces (virology), Fluorescent Antibody Technique, Genome, Viral (genetics), Host Specificity, Humans, Molecular Sequence Data, Pandemics (prevention & control), Pandemics (veterinary), Peptidyl-Dipeptidase A (genetics), Peptidyl-Dipeptidase A (metabolism), Real-Time Polymerase Chain Reaction, Receptors, Virus (genetics), Receptors, Virus (metabolism), SARS Virus (genetics), SARS Virus (isolation & purification), SARS Virus (metabolism), SARS Virus (ultrastructure), Severe Acute Respiratory Syndrome (prevention & control), Severe Acute Respiratory Syndrome (transmission), Severe Acute Respiratory Syndrome (veterinary), Severe Acute Respiratory Syndrome (virology), Species Specificity, Spike Glycoprotein, Coronavirus (chemistry), Spike Glycoprotein, Coronavirus (metabolism), Vero Cells, Virion (isolation & purification), Virion (ultrastructure), Virus Internalization, Viverridae (metabolism).
- MESH :
- chemical , chemistry : Spike Glycoprotein, Coronavirus.
- chemical , genetics : Peptidyl-Dipeptidase A, Receptors, Virus.
- chemical , metabolism : Peptidyl-Dipeptidase A, Receptors, Virus, Spike Glycoprotein, Coronavirus.
- geographic : China.
- genetics : Genome, Viral, SARS Virus.
- isolation & purification : SARS Virus, Virion.
- metabolism : SARS Virus, Viverridae.
- prevention & control : Pandemics, Severe Acute Respiratory Syndrome.
- transmission : Severe Acute Respiratory Syndrome.
- ultrastructure : SARS Virus, Virion.
- veterinary : Pandemics, Severe Acute Respiratory Syndrome.
- virology : Chiroptera, Disease Reservoirs, Feces, Severe Acute Respiratory Syndrome.
- Animals, Chlorocebus aethiops, Fluorescent Antibody Technique, Host Specificity, Humans, Molecular Sequence Data, Real-Time Polymerase Chain Reaction, Species Specificity, Vero Cells, Virus Internalization.
Abstract
The 2002-3 pandemic caused by severe acute respiratory syndrome coronavirus (SARS-CoV) was one of the most significant public health events in recent history. An ongoing outbreak of Middle East respiratory syndrome coronavirus suggests that this group of viruses remains a key threat and that their distribution is wider than previously recognized. Although bats have been suggested to be the natural reservoirs of both viruses, attempts to isolate the progenitor virus of SARS-CoV from bats have been unsuccessful. Diverse SARS-like coronaviruses (SL-CoVs) have now been reported from bats in China, Europe and Africa, but none is considered a direct progenitor of SARS-CoV because of their phylogenetic disparity from this virus and the inability of their spike proteins to use the SARS-CoV cellular receptor molecule, the human angiotensin converting enzyme II (ACE2). Here we report whole-genome sequences of two novel bat coronaviruses from Chinese horseshoe bats (family: Rhinolophidae) in Yunnan, China: RsSHC014 and Rs3367. These viruses are far more closely related to SARS-CoV than any previously identified bat coronaviruses, particularly in the receptor binding domain of the spike protein. Most importantly, we report the first recorded isolation of a live SL-CoV (bat SL-CoV-WIV1) from bat faecal samples in Vero E6 cells, which has typical coronavirus morphology, 99.9% sequence identity to Rs3367 and uses ACE2 from humans, civets and Chinese horseshoe bats for cell entry. Preliminary in vitro testing indicates that WIV1 also has a broad species tropism. Our results provide the strongest evidence to date that Chinese horseshoe bats are natural reservoirs of SARS-CoV, and that intermediate hosts may not be necessary for direct human infection by some bat SL-CoVs. They also highlight the importance of pathogen-discovery programs targeting high-risk wildlife groups in emerging disease hotspots as a strategy for pandemic preparedness.
DOI: 10.1038/nature12711
PubMed: 24172901
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 001109
- to stream PubMed, to step Curation: 001109
- to stream PubMed, to step Checkpoint: 001147
- to stream Ncbi, to step Merge: 002771
- to stream Ncbi, to step Curation: 002771
- to stream Ncbi, to step Checkpoint: 002771
- to stream Main, to step Merge: 001914
- to stream Main, to step Curation: 001908
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Isolation and characterization of a bat SARS-like coronavirus that uses the ACE2 receptor.</title><author><name sortKey="Ge, Xing Yi" sort="Ge, Xing Yi" uniqKey="Ge X" first="Xing-Yi" last="Ge">Xing-Yi Ge</name><affiliation><nlm:affiliation>1] Center for Emerging Infectious Diseases, State Key Laboratory of Virology, Wuhan Institute of Virology of the Chinese Academy of Sciences, Wuhan 430071, China [2].</nlm:affiliation><wicri:noCountry code="subField">China [2]</wicri:noCountry></affiliation></author><author><name sortKey="Li, Jia Lu" sort="Li, Jia Lu" uniqKey="Li J" first="Jia-Lu" last="Li">Jia-Lu Li</name></author><author><name sortKey="Yang, Xing Lou" sort="Yang, Xing Lou" uniqKey="Yang X" first="Xing-Lou" last="Yang">Xing-Lou Yang</name></author><author><name sortKey="Chmura, Aleksei A" sort="Chmura, Aleksei A" uniqKey="Chmura A" first="Aleksei A" last="Chmura">Aleksei A. Chmura</name></author><author><name sortKey="Zhu, Guangjian" sort="Zhu, Guangjian" uniqKey="Zhu G" first="Guangjian" last="Zhu">Guangjian Zhu</name></author><author><name sortKey="Epstein, Jonathan H" sort="Epstein, Jonathan H" uniqKey="Epstein J" first="Jonathan H" last="Epstein">Jonathan H. Epstein</name></author><author><name sortKey="Mazet, Jonna K" sort="Mazet, Jonna K" uniqKey="Mazet J" first="Jonna K" last="Mazet">Jonna K. Mazet</name></author><author><name sortKey="Hu, Ben" sort="Hu, Ben" uniqKey="Hu B" first="Ben" last="Hu">Ben Hu</name></author><author><name sortKey="Zhang, Wei" sort="Zhang, Wei" uniqKey="Zhang W" first="Wei" last="Zhang">Wei Zhang</name></author><author><name sortKey="Peng, Cheng" sort="Peng, Cheng" uniqKey="Peng C" first="Cheng" last="Peng">Cheng Peng</name></author><author><name sortKey="Zhang, Yu Ji" sort="Zhang, Yu Ji" uniqKey="Zhang Y" first="Yu-Ji" last="Zhang">Yu-Ji Zhang</name></author><author><name sortKey="Luo, Chu Ming" sort="Luo, Chu Ming" uniqKey="Luo C" first="Chu-Ming" last="Luo">Chu-Ming Luo</name></author><author><name sortKey="Tan, Bing" sort="Tan, Bing" uniqKey="Tan B" first="Bing" last="Tan">Bing Tan</name></author><author><name sortKey="Wang, Ning" sort="Wang, Ning" uniqKey="Wang N" first="Ning" last="Wang">Ning Wang</name></author><author><name sortKey="Zhu, Yan" sort="Zhu, Yan" uniqKey="Zhu Y" first="Yan" last="Zhu">Yan Zhu</name></author><author><name sortKey="Crameri, Gary" sort="Crameri, Gary" uniqKey="Crameri G" first="Gary" last="Crameri">Gary Crameri</name></author><author><name sortKey="Zhang, Shu Yi" sort="Zhang, Shu Yi" uniqKey="Zhang S" first="Shu-Yi" last="Zhang">Shu-Yi Zhang</name></author><author><name sortKey="Wang, Lin Fa" sort="Wang, Lin Fa" uniqKey="Wang L" first="Lin-Fa" last="Wang">Lin-Fa Wang</name></author><author><name sortKey="Daszak, Peter" sort="Daszak, Peter" uniqKey="Daszak P" first="Peter" last="Daszak">Peter Daszak</name></author><author><name sortKey="Shi, Zheng Li" sort="Shi, Zheng Li" uniqKey="Shi Z" first="Zheng-Li" last="Shi">Zheng-Li Shi</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2013">2013</date><idno type="RBID">pubmed:24172901</idno><idno type="pmid">24172901</idno><idno type="doi">10.1038/nature12711</idno><idno type="wicri:Area/PubMed/Corpus">001109</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001109</idno><idno type="wicri:Area/PubMed/Curation">001109</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001109</idno><idno type="wicri:Area/PubMed/Checkpoint">001147</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001147</idno><idno type="wicri:Area/Ncbi/Merge">002771</idno><idno type="wicri:Area/Ncbi/Curation">002771</idno><idno type="wicri:Area/Ncbi/Checkpoint">002771</idno><idno type="wicri:Area/Main/Merge">001914</idno><idno type="wicri:Area/Main/Curation">001908</idno><idno type="wicri:Area/Main/Exploration">001908</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Isolation and characterization of a bat SARS-like coronavirus that uses the ACE2 receptor.</title><author><name sortKey="Ge, Xing Yi" sort="Ge, Xing Yi" uniqKey="Ge X" first="Xing-Yi" last="Ge">Xing-Yi Ge</name><affiliation><nlm:affiliation>1] Center for Emerging Infectious Diseases, State Key Laboratory of Virology, Wuhan Institute of Virology of the Chinese Academy of Sciences, Wuhan 430071, China [2].</nlm:affiliation><wicri:noCountry code="subField">China [2]</wicri:noCountry></affiliation></author><author><name sortKey="Li, Jia Lu" sort="Li, Jia Lu" uniqKey="Li J" first="Jia-Lu" last="Li">Jia-Lu Li</name></author><author><name sortKey="Yang, Xing Lou" sort="Yang, Xing Lou" uniqKey="Yang X" first="Xing-Lou" last="Yang">Xing-Lou Yang</name></author><author><name sortKey="Chmura, Aleksei A" sort="Chmura, Aleksei A" uniqKey="Chmura A" first="Aleksei A" last="Chmura">Aleksei A. Chmura</name></author><author><name sortKey="Zhu, Guangjian" sort="Zhu, Guangjian" uniqKey="Zhu G" first="Guangjian" last="Zhu">Guangjian Zhu</name></author><author><name sortKey="Epstein, Jonathan H" sort="Epstein, Jonathan H" uniqKey="Epstein J" first="Jonathan H" last="Epstein">Jonathan H. Epstein</name></author><author><name sortKey="Mazet, Jonna K" sort="Mazet, Jonna K" uniqKey="Mazet J" first="Jonna K" last="Mazet">Jonna K. Mazet</name></author><author><name sortKey="Hu, Ben" sort="Hu, Ben" uniqKey="Hu B" first="Ben" last="Hu">Ben Hu</name></author><author><name sortKey="Zhang, Wei" sort="Zhang, Wei" uniqKey="Zhang W" first="Wei" last="Zhang">Wei Zhang</name></author><author><name sortKey="Peng, Cheng" sort="Peng, Cheng" uniqKey="Peng C" first="Cheng" last="Peng">Cheng Peng</name></author><author><name sortKey="Zhang, Yu Ji" sort="Zhang, Yu Ji" uniqKey="Zhang Y" first="Yu-Ji" last="Zhang">Yu-Ji Zhang</name></author><author><name sortKey="Luo, Chu Ming" sort="Luo, Chu Ming" uniqKey="Luo C" first="Chu-Ming" last="Luo">Chu-Ming Luo</name></author><author><name sortKey="Tan, Bing" sort="Tan, Bing" uniqKey="Tan B" first="Bing" last="Tan">Bing Tan</name></author><author><name sortKey="Wang, Ning" sort="Wang, Ning" uniqKey="Wang N" first="Ning" last="Wang">Ning Wang</name></author><author><name sortKey="Zhu, Yan" sort="Zhu, Yan" uniqKey="Zhu Y" first="Yan" last="Zhu">Yan Zhu</name></author><author><name sortKey="Crameri, Gary" sort="Crameri, Gary" uniqKey="Crameri G" first="Gary" last="Crameri">Gary Crameri</name></author><author><name sortKey="Zhang, Shu Yi" sort="Zhang, Shu Yi" uniqKey="Zhang S" first="Shu-Yi" last="Zhang">Shu-Yi Zhang</name></author><author><name sortKey="Wang, Lin Fa" sort="Wang, Lin Fa" uniqKey="Wang L" first="Lin-Fa" last="Wang">Lin-Fa Wang</name></author><author><name sortKey="Daszak, Peter" sort="Daszak, Peter" uniqKey="Daszak P" first="Peter" last="Daszak">Peter Daszak</name></author><author><name sortKey="Shi, Zheng Li" sort="Shi, Zheng Li" uniqKey="Shi Z" first="Zheng-Li" last="Shi">Zheng-Li Shi</name></author></analytic><series><title level="j">Nature</title><idno type="eISSN">1476-4687</idno><imprint><date when="2013" type="published">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>China</term><term>Chiroptera (virology)</term><term>Chlorocebus aethiops</term><term>Disease Reservoirs (virology)</term><term>Feces (virology)</term><term>Fluorescent Antibody Technique</term><term>Genome, Viral (genetics)</term><term>Host Specificity</term><term>Humans</term><term>Molecular Sequence Data</term><term>Pandemics (prevention & control)</term><term>Pandemics (veterinary)</term><term>Peptidyl-Dipeptidase A (genetics)</term><term>Peptidyl-Dipeptidase A (metabolism)</term><term>Real-Time Polymerase Chain Reaction</term><term>Receptors, Virus (genetics)</term><term>Receptors, Virus (metabolism)</term><term>SARS Virus (genetics)</term><term>SARS Virus (isolation & purification)</term><term>SARS Virus (metabolism)</term><term>SARS Virus (ultrastructure)</term><term>Severe Acute Respiratory Syndrome (prevention & control)</term><term>Severe Acute Respiratory Syndrome (transmission)</term><term>Severe Acute Respiratory Syndrome (veterinary)</term><term>Severe Acute Respiratory Syndrome (virology)</term><term>Species Specificity</term><term>Spike Glycoprotein, Coronavirus (chemistry)</term><term>Spike Glycoprotein, Coronavirus (metabolism)</term><term>Vero Cells</term><term>Virion (isolation & purification)</term><term>Virion (ultrastructure)</term><term>Virus Internalization</term><term>Viverridae (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Cellules Vero</term><term>Chine</term><term>Chiroptera (virologie)</term><term>Données de séquences moléculaires</term><term>Fèces (virologie)</term><term>Glycoprotéine de spicule des coronavirus ()</term><term>Glycoprotéine de spicule des coronavirus (métabolisme)</term><term>Génome viral (génétique)</term><term>Humains</term><term>Pandémies ()</term><term>Pandémies (médecine vétérinaire)</term><term>Peptidyl-Dipeptidase A (génétique)</term><term>Peptidyl-Dipeptidase A (métabolisme)</term><term>Pénétration virale</term><term>Réaction de polymérisation en chaine en temps réel</term><term>Récepteurs viraux (génétique)</term><term>Récepteurs viraux (métabolisme)</term><term>Réservoirs d'agents pathogènes (virologie)</term><term>Spécificité d'espèce</term><term>Spécificité d'hôte</term><term>Syndrome respiratoire aigu sévère ()</term><term>Syndrome respiratoire aigu sévère (médecine vétérinaire)</term><term>Syndrome respiratoire aigu sévère (transmission)</term><term>Syndrome respiratoire aigu sévère (virologie)</term><term>Technique d'immunofluorescence</term><term>Virion (isolement et purification)</term><term>Virion (ultrastructure)</term><term>Virus du SRAS (génétique)</term><term>Virus du SRAS (isolement et purification)</term><term>Virus du SRAS (métabolisme)</term><term>Virus du SRAS (ultrastructure)</term><term>Viverridae (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Peptidyl-Dipeptidase A</term><term>Receptors, Virus</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Peptidyl-Dipeptidase A</term><term>Receptors, Virus</term><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>China</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Genome, Viral</term><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Génome viral</term><term>Peptidyl-Dipeptidase A</term><term>Récepteurs viraux</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>SARS Virus</term><term>Virion</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Virion</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>SARS Virus</term><term>Viverridae</term></keywords><keywords scheme="MESH" qualifier="médecine vétérinaire" xml:lang="fr"><term>Pandémies</term><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Glycoprotéine de spicule des coronavirus</term><term>Peptidyl-Dipeptidase A</term><term>Récepteurs viraux</term><term>Virus du SRAS</term><term>Viverridae</term></keywords><keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Pandemics</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="transmission" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="en"><term>SARS Virus</term><term>Virion</term></keywords><keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>Pandemics</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Chiroptera</term><term>Fèces</term><term>Réservoirs d'agents pathogènes</term><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Chiroptera</term><term>Disease Reservoirs</term><term>Feces</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Chlorocebus aethiops</term><term>Fluorescent Antibody Technique</term><term>Host Specificity</term><term>Humans</term><term>Molecular Sequence Data</term><term>Real-Time Polymerase Chain Reaction</term><term>Species Specificity</term><term>Vero Cells</term><term>Virus Internalization</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cellules Vero</term><term>Chine</term><term>Données de séquences moléculaires</term><term>Glycoprotéine de spicule des coronavirus</term><term>Humains</term><term>Pandémies</term><term>Pénétration virale</term><term>Réaction de polymérisation en chaine en temps réel</term><term>Spécificité d'espèce</term><term>Spécificité d'hôte</term><term>Syndrome respiratoire aigu sévère</term><term>Technique d'immunofluorescence</term><term>Virion</term><term>Virus du SRAS</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>République populaire de Chine</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The 2002-3 pandemic caused by severe acute respiratory syndrome coronavirus (SARS-CoV) was one of the most significant public health events in recent history. An ongoing outbreak of Middle East respiratory syndrome coronavirus suggests that this group of viruses remains a key threat and that their distribution is wider than previously recognized. Although bats have been suggested to be the natural reservoirs of both viruses, attempts to isolate the progenitor virus of SARS-CoV from bats have been unsuccessful. Diverse SARS-like coronaviruses (SL-CoVs) have now been reported from bats in China, Europe and Africa, but none is considered a direct progenitor of SARS-CoV because of their phylogenetic disparity from this virus and the inability of their spike proteins to use the SARS-CoV cellular receptor molecule, the human angiotensin converting enzyme II (ACE2). Here we report whole-genome sequences of two novel bat coronaviruses from Chinese horseshoe bats (family: Rhinolophidae) in Yunnan, China: RsSHC014 and Rs3367. These viruses are far more closely related to SARS-CoV than any previously identified bat coronaviruses, particularly in the receptor binding domain of the spike protein. Most importantly, we report the first recorded isolation of a live SL-CoV (bat SL-CoV-WIV1) from bat faecal samples in Vero E6 cells, which has typical coronavirus morphology, 99.9% sequence identity to Rs3367 and uses ACE2 from humans, civets and Chinese horseshoe bats for cell entry. Preliminary in vitro testing indicates that WIV1 also has a broad species tropism. Our results provide the strongest evidence to date that Chinese horseshoe bats are natural reservoirs of SARS-CoV, and that intermediate hosts may not be necessary for direct human infection by some bat SL-CoVs. They also highlight the importance of pathogen-discovery programs targeting high-risk wildlife groups in emerging disease hotspots as a strategy for pandemic preparedness. </div></front></TEI><affiliations><list></list><tree><noCountry><name sortKey="Chmura, Aleksei A" sort="Chmura, Aleksei A" uniqKey="Chmura A" first="Aleksei A" last="Chmura">Aleksei A. Chmura</name><name sortKey="Crameri, Gary" sort="Crameri, Gary" uniqKey="Crameri G" first="Gary" last="Crameri">Gary Crameri</name><name sortKey="Daszak, Peter" sort="Daszak, Peter" uniqKey="Daszak P" first="Peter" last="Daszak">Peter Daszak</name><name sortKey="Epstein, Jonathan H" sort="Epstein, Jonathan H" uniqKey="Epstein J" first="Jonathan H" last="Epstein">Jonathan H. Epstein</name><name sortKey="Ge, Xing Yi" sort="Ge, Xing Yi" uniqKey="Ge X" first="Xing-Yi" last="Ge">Xing-Yi Ge</name><name sortKey="Hu, Ben" sort="Hu, Ben" uniqKey="Hu B" first="Ben" last="Hu">Ben Hu</name><name sortKey="Li, Jia Lu" sort="Li, Jia Lu" uniqKey="Li J" first="Jia-Lu" last="Li">Jia-Lu Li</name><name sortKey="Luo, Chu Ming" sort="Luo, Chu Ming" uniqKey="Luo C" first="Chu-Ming" last="Luo">Chu-Ming Luo</name><name sortKey="Mazet, Jonna K" sort="Mazet, Jonna K" uniqKey="Mazet J" first="Jonna K" last="Mazet">Jonna K. Mazet</name><name sortKey="Peng, Cheng" sort="Peng, Cheng" uniqKey="Peng C" first="Cheng" last="Peng">Cheng Peng</name><name sortKey="Shi, Zheng Li" sort="Shi, Zheng Li" uniqKey="Shi Z" first="Zheng-Li" last="Shi">Zheng-Li Shi</name><name sortKey="Tan, Bing" sort="Tan, Bing" uniqKey="Tan B" first="Bing" last="Tan">Bing Tan</name><name sortKey="Wang, Lin Fa" sort="Wang, Lin Fa" uniqKey="Wang L" first="Lin-Fa" last="Wang">Lin-Fa Wang</name><name sortKey="Wang, Ning" sort="Wang, Ning" uniqKey="Wang N" first="Ning" last="Wang">Ning Wang</name><name sortKey="Yang, Xing Lou" sort="Yang, Xing Lou" uniqKey="Yang X" first="Xing-Lou" last="Yang">Xing-Lou Yang</name><name sortKey="Zhang, Shu Yi" sort="Zhang, Shu Yi" uniqKey="Zhang S" first="Shu-Yi" last="Zhang">Shu-Yi Zhang</name><name sortKey="Zhang, Wei" sort="Zhang, Wei" uniqKey="Zhang W" first="Wei" last="Zhang">Wei Zhang</name><name sortKey="Zhang, Yu Ji" sort="Zhang, Yu Ji" uniqKey="Zhang Y" first="Yu-Ji" last="Zhang">Yu-Ji Zhang</name><name sortKey="Zhu, Guangjian" sort="Zhu, Guangjian" uniqKey="Zhu G" first="Guangjian" last="Zhu">Guangjian Zhu</name><name sortKey="Zhu, Yan" sort="Zhu, Yan" uniqKey="Zhu Y" first="Yan" last="Zhu">Yan Zhu</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001908 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001908 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:24172901
|texte= Isolation and characterization of a bat SARS-like coronavirus that uses the ACE2 receptor.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:24172901" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a SrasV1
| This area was generated with Dilib version V0.6.33. |  |